![]() ![]() We will use the images NeuralTube.jpg plus NeuralTube Blue Saturated.tif and TrichromeIHC.tif throughout this section, along with the image set ‘RGB- blue.tif, RGB-green.tif and RGB-red.tif'. This section will show you how to arrange and alter multi-channel images and change the colour of your images. Often you will want to add colour to each channel (usually in the colour of the fluorophores emission) and to create composite images of 2 or more of the channels combined. This means that each fluorophore is captured as a different image with no associated colour. ![]() Please contact the moderators of this subreddit if you have any questions or concerns.Immunofluorescence experiments are almost always captured on highly sensitive monochrome cameras. I am a bot, and this action was performed automatically.
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